11/7/2022 0 Comments Pam karlson chicago“He had this really characteristic twinkle in his eye and he would smirk and say, ‘You’re just a specialized veterinarian.’”Ĭourtney Sakas noted that she had a special appreciation for her father because he gave “patients a voice that didn’t have one.” “In a teasing way, I would say, ‘There’s a real doctor in the family - I take care of people,’” she said. His CD and record collection was immense, family members said.ĭaughter Courtney Sakas said she and her father would joke about her decision to become a physician. (Jim Sakas)įriends and family recalled Sakas’ dry sense of humor, his compassion for people and animals alike, and his love of music, everything from opera to classic rock. Peter Sakas, cousin Peter Sakas, sister Connie Markoutsas, and brothers Bill and Jim Sakas. “He was literally the foundation of our organization,” Garrido said. Last year, the foundation rescued nearly 300 dogs and about a dozen cats, nearly all of which Sakas would see and evaluate, Garrido said. John Garrido, founder of Garrido Stray Rescue Foundation, said he turned to Sakas to care for the many stray dogs and other animals his organization took in off the streets. “Pete was probably the only one who ever put braces on a bird!” “Six weeks later, we took the braces off and the bird was healed,” she said. #PAM KARLSON CHICAGO HOW TO#The pair figured out how to bond orthodontic braces with rubber bands to the outside of the bird’s beak, Rosen said. Sakas’ sister-in-law, orthodontist Jackie Rosen, recalled how Sakas consulted with her many years ago on an exotic bird with a broken beak who could no longer eat. “I felt he always went the extra mile with the care of our pets,” said Karlson, who also worked with Sakas as a volunteer of Flint Creek Wildlife Rehabilitation. She continued to see him when she added cats to her home through the years. Pam Karlson, of Chicago, visited Sakas for veterinarian services for 35 years, starting when her future husband bought her a parakeet shortly after college. It turned out it was benign, the hawk made a recovery and was released.” (Sakas) did brilliant surgery to remove the mass. “We took in a really sick red-tailed, adult male hawk that had a huge mass on his neck. “He saw the cases that were the toughest, that were beyond what we could do without him,” Keller said. In the mid-2000s, Sakas formed a partnership with Barrington-based Flint Creek Wildlife Rehabilitation, an organization that provides medical care to injured wildlife and releases the animals back into their habitats when they are well again, said founder and director Dawn Keller. “He helped dogs, cats, birds, hawks, deer, geese. “He took care of everything,” Markoutsas said. Sakas was known for his care of birds, from parakeets and parrots to wild pigeons and hawks, but he would treat just about any animal brought before him, those who knew him said. “He was able to do good and he did it effortlessly because that is the person he was.” “He was a vet in the same way he was a person: caring and compassionate,” said Al Whitman, who attended veterinarian school with Sakas at the University of Illinois. He frequently took on cases that others considered helpless and he would often double-book himself or come in to the clinic after hours to care for the animals, both pets and wildlife, he so dearly loved.” “He practiced medicine in a manner that was testament to those beliefs. This review highlights the progression of CRISPR/dCas9 technology as well as its applications and potential challenges in crop improvement.“He truly believed all life is precious and that animals have souls,” they wrote. Despite its potential applications and benefits, the challenges and limitations faced by the CRISPR/dCas9 system include the off-target effects, protospacer adjacent motif (PAM) sequence requirements, efficient delivery methods and the CRISPR/dCas9-interfered crops being labeled as genetically modified organisms in several countries. The CRISPR/dCas9 system has been explored as a tool for transcriptional modulation and genome imaging. The binding of the dCas9/sgRNA complex to a target gene results in transcriptional interference. This type of dCas9 is incapable to cleave DNA but retains its ability to specifically bind to DNA. Deriving from the CRISPR/Cas9 system, both systems consist of a catalytically dead Cas9 (dCas9), a transcriptional effector and a single guide RNA (sgRNA). RNA-guided genomic transcriptional regulation tools, namely clustered regularly interspaced short palindromic repeats interference (CRISPRi) and CRISPR-mediated gene activation (CRISPRa), are a powerful technology for gene functional studies.
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